We are interested in how folate mediated one-carbon metabolism contributes to malignant tumor onset andprogression in the context of glioblastoma.
Recently, we demonstrated in vitroand in vivo that cancer cells haveincreased rates of serine catabolism that exceed the anabolic one-
carbon demandfor proliferation. The uncovered pathway is characterized by excessive productionof formate, an end-product of serine catabolism.
Furthermore, we observed thathigh formate concentrations promote the invasiveness of glioblastoma cells in agrowth independent manner (Meiser et al.
2016, Science Advances; Meiser et al., 2018, Nature Communications).
The aim of this PhD project will be tounderstand the role of serine catabolism with formate overflow in tumorassociated macrophages and its impact on tumor progression.
The successful candidate will be embedded in a state-of-the-art laboratoryat the Department of Oncology composed of more than 85 staff members.
Your workwill be mainly focused on in vitroassays and will be complemented with targeted in vivo experiments using clinical relevant brain tumor mousemodels.
Trained personnel will be available for the execution of the in vivo work, a personal animal licensemay be acquired upon interest (but is not a must).
We will also take advantageof the state-of-the-art Mass Spectrometry infrastructure to run the generatedmetabolite samples.
The candidate will investigate how serine catabolism impacts the immuneresponse of tumor associated macrophages and their interplay with cancer cells,using a wide range of tools from the area of cell biology, molecular biologyand biochemistry (including stable-
isotope assisted metabolic flux analysis). Excellenttraining opportunities will be available.
KEY SKILLS, EXPERIENCE AND QUALIFICATIONS
The project can be a promising entry point to progress yourcareer development towards becoming an independent researcher in the fields ofglioma biology and / or cancer (immuno)metabolism.
Applications including cover letter, CV, list of publications and the name of two referees should be sent before 15 September 2018 through the "apply" button below ONLY